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Animal Reproduction and Genetics Programme

OBJECTIVES 

General

Conduct research to improve reproductive efficiency and genetics of food animals

Specific

  • Semen preservation technology in buffalo and local cattle.
  • Oestrus control and timed AI in buffalo and local cattle
  • Genetic improvement of indigenous poultry

FACILITIES 

  • State-of-the-art technique is available for semen collection, evaluation and cryopreservation
  • Reproductive ultrasonography
  • In vitro fertilization laboratory
  • Hormone analysis (ELISA)

SERVICES 

  • Supervise research of postgraduate students (M.Sc, M.Phil. and Ph.D).
  • Training of community workers in the techniques of artificial insemination and pregnancy diagnosis in cows and buffaloesis being done in collaboration with Agricultural Polytechnic. Institute of NARC.

CURRENT RESEARCH 

  • Improving reproductive efficiency of cows and buffaloes through oestrus synchronization and timed artificial insemination(ALP Project)
  • Investigations on Fertility-Related Biomarkers in Buffalo Semen to Reduce Male-Factor Losses (PSF Project)
  • Genetic Improvement of selected indigenous naked neck by crossing with exotic poultry breeds of Rhode Island Red and Black Australop (ALP project).

RESEARCH ACHIEVEMENTS 

Multiple ovulation and embryo transfer (MOET)

First embryo transfer calf in Pakistan was born at NARC in 1987 followed by first embryo transfer lamb in 1992. The technique has not been so far applied in buffalo due to poor embryo recovery. However, application of ultra sound guided ovum pick up technique and IVF may help in in vitro embryo production in buffalo.

Semen preservation and artificial insemination

  • Tris–citric acid based extender was tested to replace the conventional Na-citrate buffer for the freezing of buffalo and cattle semen. An improved conception rate (53% vs 29%) was achieved in the field trials with semen frozen in Tris–citric acid extender.
  • Chilled (5°C) semen is another available technique for AI. Research in this area showed that skim milk is the best preservative among five different extenders including cow milk, buffalo milk, camel milk and homogenized UHT milk.
  • Addition of antioxidants in extender improved the quality of buffalo semen processed for cryopreservation.
  • Thawing and carrying semen in ice water, a user friendly semen carrying method was being practiced by field technicians resulted in 20% decline in fertility when inseminations were made after holding semen for 30 to 60 min in ice water in buffalo.
  • Addition of antibiotic combination gentamycin, tylosine and linco-spectin to extender resulted  in improved post thaw quality and fertility of Sahiwal and Buffalo bull spermatozoa.

Pregnancy test in sheep/goat using ultrasonography

Pregnancy test procedure with ultrasonography was standardized in local sheep. Accurate pregnancy diagnosis in Balkhi ewes was achieved at day 42 of gestation by transabdominal ultrasonography using a 3.5 MHz probe. 

Summer anoestrous in buffalo

Progesterone implant was tested to induce heat in buffaloes during low breeding season (April May). Heat signs were noted in 80% animals after withdrawal of implant. A conception rate of 30-40% was observed at first service immediately after hormone withdrawal.

Artificial insemination in goat

AI in goats was introduced successfully for genetic improvement of non descript breeds. In this regard following techniques were standardized:

  • Semen collection  and preservation
  • Oestrous synchronization  and timed AI

Kids born through artificial insemination in goats

Genetic trend in large ruminants

Genetic trend was estimated for the first time in Nili Ravi buffalo, Tharpakar and Holstein Friesian cows. The genetic trends for different provinces/breeds are given below:

Punjab (Nili Ravi Buffalo)

 

Sindh (Tharparkar Cattle­)

 

KPK (Holstein Friesian cows)

 

Baluchistan (Holstein Friesian cows)

 

Genetic improvement of Kundhi buffalo

  • A nucleus herd of 60 buffaloes (bull mothers) was established at Kundhi buffalo Farm after analysis of 28 years production record.

Integrated Approach for Improving Small Scale Market Oriented Dairy Systems in Pakistan:

  • An economic opportunity survey of dairy farmers revealed that maximum benefit is from an increase in milk production which is the combined effect of nutrition, reproduction and genetics.
  • Interventions for controlling sub clinical mastitis, calf mortality and vaccination against infectious diseases implemented at farmers herds resulted in decreased calf mortality (42%vs 5%), subclinical mastitis (36% vs 5%) and infections.

 

PUBLICATIONS

 

SCIENTIFIC STAFF

Sr. #

Name

Designation

Contact No.

02

Dr. Abdul Ghaffar

PSO/PI

90733859

03

Dr. S. M. H. Andrabi

PSO

90733858

 

Resource Person:

Dr. Abdul Ghaffar

PSO/ Program Incharge

Email/ Contact: 

051-90733859

 

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